Alpha Tubulin Molecular Weight Santa Cruz
The target antigen may refer to the tubulin polymer that comprises microtubules of the cytoskeleton as well as individual members of the tubulin protein superfamily.
Alpha tubulin molecular weight santa cruz. Anti tubulin antibody products from santa cruz biotechnology inc. α and β tubulins form heterodimers which multimerize to form a microtubule filament. After electrophoresis the blot was stained with bodipy tr x succinimidyl ester red signal to detect total protein. Antigen retrieval was performed by heat induction in citrate buffer ph 6.
α tubulin antibody tu 02 is a mouse monoclonal igm kappa light chain provided at 200 µg ml. Sc 5286 santa cruz biotechnology inc. Raised against amino acids 1 451 representing full length α tubulin of porcine origin. Samples of protein molecular weight standards product p 6649 containing decreasing amounts of a tubulin were run on an sds polyacrylamide gel and blotted onto a pvdf membrane.
Ab4074 was tested in a tissue microarray tma containing a wide range of normal and cancer tissues as well as a. Tubulin antibody sigma monoclonal anti α tubulin antibody produced in mouse. Target information alpha tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. Alpha beta tubulin heterodimer is the basic building block of microtubules and this intracellular.
Radioimmunoassay indirect immunofluorescence cultured human or chicken fibroblasts 1 2 000 western blot human fibroblast cell extract 1 4 000. Sc 8035 santa cruz biotechnology inc. These maintain cell structure provide platforms for intracellular transport and are also involved in cell division. There are a number of different subtypes that have a molecular weight of 50kda and are able to bind to beta tubulin forming a heterodimer that polymerizes to microtubules as part of the cytoskeleton.
Also reactive with additional species including and canine bovine and porcine. Santa cruz biotechnology inc. Anti alpha tubulin antibody tu 02 is recommended for detection of α tubulin of mouse rat human and porcine origin by wb ip if ihc p fcm and elisa. Anti alpha tubulin antibody b 7 is recommended for detection of α tubulin of mouse rat and human origin by wb ip if ihc p fcm and elisa.
Ab4074 staining alpha tubulin in the glandular cells of the endometrium. Pa5 19489 detects alpha tubulin which has a predicted molecular weight of approximately 50 kda. Alpha tubulin belongs to a large superfamily of tubulin proteins. α and β tubulins form heterodimers which multimerize to form a microtubule.
Alpha tubulin antibody sigma anti tubulin sigma tubulin antibody sigma. Raised against amino acids 149 448 of α tubulin of human origin. Santa cruz biotechnology inc. After staining the blot was incubated with a mouse monoclonal anti a tubulin antibody.
The alpha and beta tubulins which are each about 55 kda mw are homologous but not identical.