Alpha Tubulin Molecular Weight Western Blot

By using a western blot researchers are able to identify specific proteins from a complex mixture of proteins. Alpha tubulin antibody dm1a nb100 690 analysis of hela and cos 7 lysates. Alexa flour goat polyclonal to rabbit igg 700 at 1 10000 dilution predicted band size.

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Cst A B Tubulin Antibody

Hrp Anti Alpha Tubulin Antibody Dm1a Ab40742 Abcam

Human Mouse Rat Alpha Tubulin Antibody Mab9344 100 R D Systems

Cst A Tubulin Dm1a Mouse Mab

Anti Alpha Tubulin Antibody Gt114 Gtx628802 Genetex

Cst A Tubulin Antibody

Anti alpha tubulin antibody dm1a loading control ab7291 at 1 2500 dilution.

Alpha tubulin molecular weight western blot. Anti alpha tubulin antibody microtubule marker ab18251 at 0 5 µg ml lane 1. Tuba4a belongs to the subfamily of tubulin which is the major building block of microtubules. 50 kda additional. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase.

The gene codes for a member of the α tubulin family and contains 448 amino acids. Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter with a theoretical molecular weight of 50 kda. α tubulin also called tubulin α 4a tuba4a is mapped to human chromosome 2q35. Western blot analysis of alpha tubulin was performed by loading 20 µg of a431 lane1 raji lane2 mda mb 231 lane3 hct 116 lane4 hela lane5 du 145 lane6 pc 3 lane7 and caco 2 lane8 cell lysate using novex nupage 12 bis tris gel product np0341box xcell surelock electrophoresis system product ei0002 novex sharp pre stained protein standard lc5800 and iblot.

Hela lysate lane 2. Western blot anti alpha tubulin antibody microtubule marker ab18251 all lanes. The α subunit of tubulin has molecular weight of 50 000. Western blot anti alpha tubulin antibody dm1a loading control ab7291 this image is courtesy of an abreview submitted by elena kashuba.

Tuba4a belongs to the subfamily of tubulin which is the major building block of microtubules. α tubulin also called tubulin α 4a tuba4a is mapped to human chromosome 2q35. 30 ugs of protein samples were used for this western blot to detect tubulin in pc3 cell lysates. Western blotting is an important technique used in cell and molecular biology.

A431 lysate lysates proteins at 20 µg per lane. Secondary all lanes. α subunit of tubulin has molecular weight of 50 000. I observed a very strong band using anti tubulin antibody around 55kd molecular weight.

Tyrosine ligase adds a c terminal tyrosine to monomeric alpha tubulin. The gene codes for a member of the α tubulin family and contains 448 amino acids. Alpha tubulin molecular weight. 50 kda observed band size.

α subunit of tubulin has molecular weight of 50 000.