Alpha Tubulin Molecular Weight Kda

50 kda and share considerable homology. The gene codes for a member of the α tubulin family and contains 448 amino acids. In addition to α and β tubulin several other tubulins have been identified bringing the number of distinct tubulin classes to seven.

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Cst A Tubulin Antibody

Hrp Anti Alpha Tubulin Antibody Dm1a Ab40742 Abcam

Anti Alpha Tubulin Antibody Gt114 Gtx628802 Genetex

Anti Alpha Tubulin Antibody Loading Control Ab89984 Is Not Available

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Anti Alpha Tubulin Antibody Loading Control Ab4074 Abcam

Electrotransfer to nitrocellulose membrane.

Alpha tubulin molecular weight kda. Belongs to the. α subunit of tubulin has molecular weight of 50 000. Volumes are for 10 cm x 10 cm. Tuba4a belongs to the subfamily of tubulin which is the major building block of microtubules.

Tubulin is the major constituent of microtubules. Each has a molecular weight of approximately 50 kda. Detects a band of approximately 50 kda predicted molecular weight. It binds two moles of gtp one at an exchangeable site on the beta chain and one at a non exchangeable site on the alpha chain.

Pa5 19489 detects alpha tubulin which has a predicted molecular weight of approximately 50 kda. Tubulin is the major constituent of microtubules. Use at an assay dependent concentration. Detects a band of approximately 50 kda predicted molecular weight.

The α subunit of tubulin has molecular weight of 50 000. Membrane blocking and antibody incubations. The β tubulin subunit is exposed on the plus end of the microtubule while the α tubulin subunit is exposed on the minus end. 50 kda can be blocked with human beta tubulin peptide ab20775.

Detects a band of approximately 51 kda predicted molecular weight. Use a concentration of 1 µg ml. α tubulin also called tubulin α 4a tuba4a is mapped to human chromosome 2q35. To form microtubules the dimers of α and β tubulin bind to gtp and assemble onto the ends of microtubules while in the gtp bound state.

It binds two moles of gtp one at an exchangeable site on the beta chain and one at a non exchangeable site on the alpha chain. Perform heat mediated antigen retrieval before commencing with ihc staining protocol. Tubulin is a heterodimer that consists of α tubulin and β tubulin. Use a concentration of 0 5 µg ml.

Both subunits have a molecular weight of approx. Abcam recommends using milk as the blocking agent. Can be blocked with alpha tubulin peptide ab23537. Detects a band of approximately 55 kda predicted molecular weight.

Prestained protein marker broad range 11 190 kda. Loading of prestained molecular weight markers 13953 5 µl lane to verify electrotransfer and biotinylated protein ladder 7727 10 µl lane to determine molecular weights are recommended. Use a concentration of 1 µg ml.